Stereotactic biopsy is a precise method of sampling a small region of brain tissue using image-guidance and minimally invasive techniques. Through only a skin puncture and tiny bony opening, an instrument is accurately passed into a brain lesion in order to determine its nature. This approach is often used to diagnose brain tumors or other disorders. CT or MRI guidance is used. Intraoperative CT Scan for Stereotactic Surgery at the University of Pittsburgh.